M. Camilles, S. Link, A. Krushelnitsky, J. Balbach, K. Saalwächter
Institute of Physics, Martin Luther University Halle-Wittenberg, Germany
Crystallins are the major vision-related (i.e. refractive) proteins found in the eye lens. The mammalian lens consist of three classes of proteins, α-, β- and γ-crystallins, the former also acts as chaperone . Commonly, proteins are subject to a continuous degradation and replacement process, but the eye lens proteins have no turnover and hence have to remain stable and soluble for a lifetime. So far, most studies have focused on single eye lens proteins and their interactions at low concentrations . Here we combine NMR and other biophysical techniques to monitor stress induced aggregation and changes of the interactions of crystallins at various concentrations . This allows us to investigate molecular effects which might lead to cataract in a highly concentrated eye lens surrounding.
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